A Rapid Procedure for the Isolation and Purification of Rat Liver Nuclear Envelope
نویسنده
چکیده
disulphide bridges in carcinoembryonic antigen (Thomas et af. , 1974). When carcinoembryonic antigen modified by treatment with 5.33rnhl-metaperiodate was hydrolysed in oxygen-free conditions and subjected to acid hydrolysis cystine was detected in a subsequent amino acid analysis, whereas only cysteic acid was obtained from carcinoembryonic antigen treated with 0.533 M-metaperiodate. Oxidative cleavage of the disulphide bonds causes changes in the tertiary structure of the protein core of carcinoembryonic antigen and thereby disorientates determinant groups, whether carbohydrate or protein, and hence decreases the ability of carcinoembryonic antigen to bind to the antiserum.
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